|
Creatinine | Dicyandiamide | Dihydrotriazine | Sodium | ||
---|---|---|---|---|---|
1 | German Manufacturer | < 50 ppm | < 20 ppm | n.d. | trace |
2 | US Manufacturer #1 | 190ppm | 400ppm | 410ppm | 750ppm |
3 | US Manufacturer #2 | 300ppm | 100ppm | 40ppm | no data |
4 | US Manufacturer #3 | 2500ppm | 300ppm | 90ppm | no data |
5 | Chinese Manufacturer #1 | 1500ppm | 2300ppm | n.d. | no data |
6 | Chinese Manufacturer #2 | 100ppm | 18000ppm | n.d. | no data |
7 | US Distributor #1 | 50ppm | 20ppm | n.d. | trace |
8 | US Distributor #2 | 520ppm | 40ppm | 24ppm | 280ppm |
9 | US Distributor #3 | 220ppm | 120ppm | 60 ppm | 1250ppm |
10 | US Distributor #4 | 3000ppm | 2000ppm | 16ppm | 220ppm |
11 | US Distributor #5 | 320ppm | 60ppm | 60ppm | no data |
12 | US Distributor #6 | 50ppm | 34000ppm! | 72ppm | 530ppm |
13 | US Distributor #7 | 70ppm | 30ppm | 300ppm | no data |
14 | US Distributor #8 | 210ppm | 80ppm | 160ppm | no data |
15 | US Distributor #9 | 50ppm | 20ppm | n.d. | trace |
16 | US Distributor #10 | 180ppm | 80ppm | 176ppm | 360ppm |
17 | US Distributor #11 | 1480ppm | 80ppm | 30ppm | no data |
18 | US Distributor #12 | 50ppm | 20ppm | n.d. | no data |
19 | GB Distributor #1 | 60ppm | 1500ppm | n.d. | no data |
20 | GB Distributor #2 | 50ppm | 20ppm | n.d. | trace |
21 | GB Distributor #3 | 80ppm | 20ppm | n.d. | no data |
22 | Spanish Distributor | 200ppm | 30ppm | n.d. | no data |
23 | Canadian Distributor | 50ppm | 20ppm | n.d. | trace |
n.d. = non-detectable
HPLC = high-performance liquid chromatography
The State of Liquid Creatines and Gels
Where do I begin with the liquid creatine issue? As you can see from the testing chart done on liquid creatine and gels, it was not a very large sample size. However, the tests were done on the better known brands of liquid creatine and gels. What if a larger sample size of liquid and gel products had been tested? I would expect to see pretty much the same results. Why? Well, even though every company selling liquid creatines and gels wants you to believe they are the one company who has discovered the magic chemical formula for keeping creatine stable in any type of liquid/gel, there is no reason at this time to believe it's true. What I do know is some of the top R&D scientists in the world have told me repeatedly that creatine will not and does not stay stable over months or years in a liquid or a gel period. I was told point blank by one of the largest creatine manufacturers in the world that they had been asked by one of the largest sports drink manufacturers in the world to design a liquid sports drink with creatine in it. They were unable to find a way to make the creatine stable in liquid no matter what they tried and lost millions in potential sales by being unable to produce the product. I suppose it's possible that some small company or entrepreneur has discovered a stabilization process that eluded one of the top laboratories in the world that works with creatine, but I highly doubt it. Now, I actually have to back up somewhat from those harsh statements about liquid creatines and gels. I was recently contacted by a scientist from a company who said they are 90% sure they will have truly stabilized liquid creatine to be launched in the near future but none of them felt anything currently on the market was stable. Our small test results would seem to agree with that assessment, but anything is possible right? As the reader can see, one gel was as low as 11.2% creatine with lots of creatinine and the liquid was only 14.4% creatine. I leave it to the reader to make up their own mind regarding such products.
HPLC Creatine Test Results (Gels and Liquids)
%Creatine | Creatinine | Dicyandiamide | Dihydrotriazine | Sodium | ||
---|---|---|---|---|---|---|
1 | Gel #1 | 14.4 | 5000 ppm | 120 ppm | n.d. | 840 ppm |
2 | Gel #2 | 11.2 | 7000 ppm | 54 ppm | n.d. | no data |
3 | Liquid | 16.8 | 5000 ppm | 570 ppm | 860 ppm | no data |
n.d. = non-detectable
HPLC = high-performance liquid chromatography
Conclusion
So what can the reader do with this information? As I stated in the first article: "As for the consumer, if it were me, I would demand the HPLC test results from whom ever I was buying my creatine from regarding the chemicals listed in this article." The same still holds true today and the tests should of course be done by an independent lab. Most companies when asked for test results on creatine will send you a simple purity test.
A purity test will tell you little to nothing. The purity level of all the creatine products were also tested and they generally fell between 88 and 92%. Now before you go off yelling "but my creatine says 99% pure creatine monohydrate on the bottle," you have to remember there is a small amount of water in creatine monohydrate which leaves plenty of space to hide impurities. So, if these impurities concern you, you should ask for the HPLC tests on those specific impurities. If you don't care about it hey, that's your business. So, more today than ever, the old expression "creatine is creatine" fails to hold water. However, a high quality creatine product it still the hottest thing going in bodybuilding/sports supplements. It increases strength, lean body mass, and, to a lesser extent, endurance, so a high quality brand of creatine is still a safe and effective supplement.
The astute reader will recall, I also mentioned I had the testing results for many pyruvate products in the beginning of the article. If you think some of the creatines are bad, wait until you see what's in your pyruvate products! That's for another article. Keep an eye out for it!
A brief description of the impurities found in low grade creatines
Dicyandiamide (DC): DC is actually a derivative of one of the starting chemicals (cyanamide) used in creatine production. DC is formed during the production of creatine products, and large amounts found in a product are considered the result of an incomplete or inefficient process. A quality creatine product will contain very small amounts, less than 20-50ppm. DC does not appear to be a particularly toxic chemical. Oral studies with animals (rats and dogs) lasting up to 90 days have not shown serious toxicity or carcinogenic effects, and acute poisoning also takes very high amounts (LD50 /oral / rats = <5000mg/kg). DC appears to have many uses in the chemical industry. Some of the more interesting is the use of DC in the production of fertilizers, explosives, fire proofing compounds, cleaning compounds, soldering compounds, stabilizer in detergents, modifier for starch products, and a catalyst for epoxy resins (AllChem Industries data sheet. AllChem Industries inc., Gainesville FL, 32607.). At the concentrations found in some of the creatine products (see below), it's a good thing this stuff does not appear to be particularly toxic. However, as far as I am concerned, I don't want to be eating the stuff.
Creatinine: Creatinine is one of the easy compounds to discuss on this list. Creatinine is actually a natural byproduct of creatine metabolism in the human body and of creatine production. A small amount can be found in every creatine product. However, in some products large amounts can be found (see chart). It is probably safe to say that the ingestion of creatinine is a safe endeavor. There is some research that links the ingestion of creatinine from meats with increased colon cancer incidence, but in all honesty I would not put much stock in that or get all worked up about it . The point is, when I buy creatine I want to eat creatine, not creatinine. Though a natural byproduct of creatine metabolism, it does not have any ergogenic effects and therefore I don't want large amounts of it in my creatine, period. A high quality creatine product should contain less than 100ppm of creatinine in my opinion.
Sodium: Like the aforementioned creatinine, sodium is an easy one to talk about. Also, like creatinine, it is a generally safe thing to ingest at normal intakes. At the levels found in these creatine products, the amount of sodium added to the diet is very small and should pose no problems, even to the most sodium phobic person. However, like I said before, when I pay for creatine I want creatine, not sodium.
Dihydrotriazine (DT): DT appears to be the real mystery chemical as far as potentially toxic contaminants found in some creatine products. One company had it listed as "...Dihydrotriazine is often found in various creatine products. This substance is a byproduct of non-optimized creatine productions and consequently widely spread over creatine products.
Dihydrotriazine is a compound with unknown pharmaceutical and toxicological properties." It was virtually impossible to find any useful safety data on this chemical. However, DT is part of a large family of chemicals known as the "triazines." It is an organic base with many derivatives. Some of these derivatives are toxic while others are known to be non-toxic, so it is very difficult to come to any real solid opinion regarding the potential toxicity of this chemical. One chemist I spoke to from a major pharmaceutical supply company said to me on the phone "it's safe to say that there will be major differences in toxicity between derivatives since 'triazine' simply means possessing three C=N-H groups. Some derivatives are highly toxic." Bill Roberts, a regular contributor to Mesomorphosis and former writer for Dan Duchaine's Dirty Dieting newsletter commented after I sent him over this information: "There really is no way to say just how high a chronic intake of this chemical [these chemicals] is safe in humans from the information given. If the amounts were very small, say a few milligrams per week, it's a reasonable guess that there would probably be no problem. But if a creatine brand has say 1% of this impurity [these impurities] then people are going to be consuming thousands of milligrams of this compound [these compounds] over time. I think we have to be concerned about taking so much of something that really isn't well studied in humans for safety. It would certainly be unwise to assume that toxicity is not an issue. If the consumer has a choice between a creatine brand that contains this impurity [these impurities] in significant amounts, and one that is more pure, I'd certainly recommend spending the extra money and obtaining the purer product."
So as you can see, we are left with a major question mark regarding DT. For me, the less I know about a chemical the less of it I want to find in any product I am ingesting. Though this chemical might turn out to be perfectly harmless, I think it should not be found in any amount and thus should be non-detectable (n.d.) in the ppm range until we know more about this chemical. As you can see from the tests, some companies have n.d. amounts while others have far more than that. I find this unacceptable, and so should you.